Survey and analysis of microsatellites from transcript sequences in Phythoptora species: frequency distribution and potential as markers for the phylum Oomycota.


Authors: Garnica D.*, Pinzon A.+, Quesada L.*, Bernal A.*, Barreto E.+, Grünwald N.J.** and Restrepo S*.


+ Centro de Bioinformatica - Instituto de Biotecnologia - Universidad Nacional de Colombia
* Laboratorio de Micologia y fitopatologia, Universidad de los Andes.
**Horticultural Crops Research Laboratory, USDA ARS, Corvallis,OR 97330
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All microsatellites were evaluated for in silico cross-transferability among all three species. In other words, one primer pair designed to amplify an SSR from one of the species, is able (making an "in silico PCR") to amplify also in another species. This primer pair would be considered as a transferable marker. The number of SSRs potentially transferable between species found in this study represents a remarkably large, diverse reservoir of markers that is potentially useful for diversity and population studies. Thus, searches and downloads can be performed by organisms between transferable primers were found, combined with the similarity alignment criterion used between primer sequences from the first organism and SSR-containing sequence from the second organism. Additionally, transferable primers between the three organisms can also be searched and downloaded from this page.


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Bogota-Colombia 2006
Ultima actualizacion Julio 17 de 2007