Survey and analysis of microsatellites from transcript sequences in Phythoptora species: frequency distribution and potential as markers for the phylum Oomycota.


Authors: Garnica D.*, Pinzon A.+, Quesada L.*, Bernal A.*, Barreto E.+, Grünwald N.J.** and Restrepo S*.


+ Centro de Bioinformatica - Instituto de Biotecnologia - Universidad Nacional de Colombia
* Laboratorio de Micologia y fitopatologia, Universidad de los Andes.
**Horticultural Crops Research Laboratory, USDA ARS, Corvallis,OR 97330
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Members of the genus Phytophthora are notorious pathogens with world-wide distribution. The most devastating species include P. infestans, P. ramorum and P. sojae.
In order to develop molecular methods for routinely characterizing their populations and to gain a better insight into the organization and evolution of their genomes, we used an in silico approach to survey simple sequence repeats (SSRs) content in transcript sequences from these three species.
Primers were designed for the SSRs detected, and all SSR-containing sequences along with their corresponding primers and a brief description of the SSR and the virtual PCR product were deposited in this database. Searches can be performed by organism in combination with SSR repeat type, motif and length. Sequences are freely accessible and can be downloaded in FASTA format.



Bogota-Colombia 2006
Ultima actualizacion Julio 17 de 2007