Nota : todas sus respuestas deben ser enviadas al siguiente email : ebarretoh@unal.edu.co.
Buena suerte.
- Con relación al alineamiento pareado cuales son la principales diferencias
entre un alineamiento global y uno local y en casos usted recomendaría utilizar
cada uno de ellos?
De manera general, como se calcula el "score" de una alineamiento
pareado?
- Las figuras de abajo son dotplots obtenidos con 2 proteínas hipotéticas
"1" and "2". La figura A representa la proteína "1" comparada contra ella
misma, La figura B representa la proteína "2" comparada contra ella misma,
y la figura C representa la proteína "1" alineada con la proteína "2".
- Porque usted obtiene una diagonal larga en la figuras A y B?
(2 pts)
- En las mismas figuras, que representan las diagonales pequeñas? (2
pts)
- Trate de deducir los posibles dominios presentes en estas dos proteínas
usando las 3 figuras (A,B,C) y haga una representación esquemática de
ellos. (6 pts)
- Debajo encontrará la salida de una búsqueda BLAST usando SwissProt y TrEMBL
como bases de datos. Este resultado fue obtenido usando el programa
blastp (protein query vs protein database) y la proteína UFD1_YEAST como
query.
- Que representan las "X" en la secuencia query? (vea el primer alineamiento)
- Cuantas proteínas en SwissProt tienen un "score" que pueda ser considerado
como significativo en esta búsqueda?
- Cual es la primera proteína con un "score" que pueda ser considerado
como significativo?
- Cual es la matriz de peso utilizada para esta búsqueda y porque?Si
pudiera escoger la matriz cual seleccionaria y porque?
- Usted desea realizar un estudio en mamíferos utilizando la siguiente secuencia de proteína que obtuvo experimentalmente con el fin de conocer su función, estructura y evolución.Para ello utilizará las herramientas bioinformáticas disponibles y que ha conocido durante el curso.
EDHVKLVNEVTEFAKKCAADESAENCDKSLHTLFGDKLCTVATLRATYGELADCCEKQEP ERNECFLTHKDDHPNLPKLKPEPDAQCAAFQEDPDKFLGKYLYEVARRHPYFYGPELLFH AEEYKADFTECCPADDKLACLIPKLDALKERILLSSAKERLKCSSFQNFGERAVKAWSVA RLSQKFPKADFAEVSKIVTDLTKVHKECCHGDLLECADDRADLAKYICEHQDSISGKLKA CCDKPLLQKSHCIAEVKEDDLPSDLPALAADFAEDKEICKHYKDAKDVFLGTFLYEYSRR HPDYSVSLLLRIAKTYEATLEKCCAEADPPACYRTVFDQFTPLVEEPKSLVKKNCDLFEE VGEYDFQNALIVRYTKKAPQVSTPTLVEIGRTLGKVGSRCCKLPESERL
Determine:
- Su función y la familia a la que pertenece
- Cual es Secuencia del gen correspondiente?
- Cuantas proteinas en SwissProt tienen un "score" que pueda ser considerado como significativo en esta búsqueda?
- Cual es la matriz de peso utilizada por defecto para la búsqueda y porque?
- Identifique en los resultados de su búsqueda un grupo de secuencias potencialmente homólogas (mínimo 8) a su query. Recuperelas, reportelas y realice un alinemiento multiple con ellas (reportelo), que le indica el alineamiento?
BLAST2.0 query receipt
Thanks. Your request has been filed with the following data:
Program:
blastp
Database: swisstrembl
Number: 4979
e-mail:
Format: SwissProt_ID
Sequence: ufd1_yeast
Processing, please wait...
blastall.remote -s
blast.cscs.ch -p blastp -d swisstrembl wwwtmp/sq.4979.txt -M blosum62
-K 0 -e 10.0 -F T -G def -E def -X def -v 50 -b 50 -g T
|
Here are your search results:
SIB BLAST network server
version 1.3 of November 15, 2000
Welcome to the SIB BLAST Network
Service (julier)
==============================================================================
Swiss Institute of Bioinformatics (SIB)
Ludwig Institute
for Cancer Research (LICR)
Swiss Institute for Experimental Cancer
Research (ISREC)
==============================================================================
If results of this search are reported or published, please mention
that
the computation was performed at the SIB using the BLAST network
service.
PEPTIDE SEQUENCE DATABASES
swiss SwissProt + updates
26-Jan-2001
swisstrembl SwissProt+Trembl+TrUpdates
26-Jan-2001
nr Non-redundant SP+Trembl+TrUpdates+gp+gpu
26-Jan-2001
gpupdate cumulative weekly updates of gp, currently
26-Jan-2001
shuffled SwissProt r30 shuffled in windows of 20 aa
yeast S.cerevisiae translated genome ORFs
NUCLEOTIDE SEQUENCE
DATABASES
embre * EMBL Data Library, Release 65, December 2000
embu * EmblUpdate 31-Jan-2001
embl
* EMBL+EmblUpdate 31-Jan-2001
EST
Database of Expressed Sequence Tags 31-Jan-2001
GSS
Genome Survey Sequences 31-Jan-2001
STS
Database of Sequence Tagged Sites 31-Jan-2001
nr *
Non-redundant EMBL+EmblUpdate+GenBank 31-Jan-2001
repbase +
Repbase, February 1996
simple Simple sequence repeats (J. Mol.
Evol. 40:120, 1995)
yeast S.cerevisiae genome
worm
C.elegans genome
* The est, gss, and sts divisions of EMBL are
excluded from these databases.
+ Contains sequences from the files
humrep.ref, invrep.ref, mamrep.ref,
plnrep.ref, rodrep.ref, and
vertrep.ref from repbase.
============================================================================
T
he BLAST Network Service uses a server developed at SIB and the NCBI
BLAST 2 software.
For problems regarding this site, please contact one
of the following persons:
- problems with the programs: Christian Iseli
(chris@cmpteam4.unil.ch)
- problems with the databases: Victor Jongeneel
(Victor.Jongeneel@isb-sib.ch)
============================================================================
BL
ASTP 2.1.2 [Oct-19-2000]
Reference: Altschul, Stephen F., Thomas L.
Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller,
and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new
generation of protein database search
programs", Nucleic Acids Res.
25:3389-3402.
Query= sp|P53044|UFD1_YEAST (UFD1..)UBIQUITIN FUSION DEGR
(361
letters)
Database: swiss_nr; swiss_varsplic_nr; trembl_new_nr;
trembl_nr;
trembl_varsplic_nr
500,285 sequences; 166,584,592
total letters
Searching..................................................done
Score E
Sequences producing significant alignments:
(bits) Value
sp|P53044|UFD1_YEAST (UFD1..)UBIQUITIN FUSION DEGRADATION PROTE... 655
0.0
tr|AJ005825|O42915 (UFD1..)UBIQUITIN FUSION DEGRADATION PROTEIN... 264
1e-69
tn|AF234601|AAG27535 UFD1.[Rattus norvegicus] 204
1e-51
sp_vs|Q92890-01|Q92890 SHORT ISOFORM OF Q92890[Homo sapiens]
203 2e-51
sp|P70362|UFD1_MOUSE (UFD1L)UBIQUITIN FUSION DEGRADATION PROTEI... 201
9e-51
tn|AF233524|AAG25922 Ubiquitin fusion degradation 1-like protei... 200
2e-50
sp|Q9VTF9|UFD1_DROME (CG6233)UBIQUITIN FUSION DEGRADATION PROTE... 196
4e-49
tr|AC006841|Q9SJV0 (AT2G21270)PUTATIVE UBIQUITIN FUSION-DEGRADA... 192
3e-48
tr|AL035679|Q9SVK0 (F19H22.30..)PUTATIVE UBIQUITIN-DEPENDENT PR... 190
2e-47
sp|Q92890|UFD1_HUMAN (UFD1L)UBIQUITIN FUSION DEGRADATION PROTEI... 185
4e-46
sp|Q19584|UFD1_CAEEL (F19B6.2)UBIQUITIN FUSION DEGRADATION PROT... 170
2e-41
tr|AC005315|O81075 (T9I4.15)PUTATIVE UBIQUITIN.[Arabidopsis tha... 169
3e-41
tr|Z69635|Q9U3I6 (F19B6.2B)F19B6.2B PROTEIN.[Caenorhabditis ele... 169
3e-41
tr|AF083031|Q9SEV9 (UFD1)UBIQUITIN FUSION DEGRADATION PROTEIN.[... 106
4e-22
tr|Z97338|O23395 (AT4G15420)SIMILAR TO UFD1 PROTEIN (UFD1 LIKE ...
102 6e-21
tn|AF315735|AAG31651 PRLI-interacting factor K (Fragment).[Arab... 102
6e-21
tr|AE001179|O51745 (BB0805)POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSF... 35
1.4
tr|AC010793|Q9MA08 F20B17.10.[Arabidopsis thaliana] 34
3.2
tr|AC010924|Q9S9M2 (T24D18.23)T24D18.23 PROTEIN.[Arabidopsis th... 34
3.2
tr|Z97182|O07779 (RV0599C..)HYPOTHETICAL 8.2 KDA PROTEIN.[Mycob... 34
3.2
tr|AC013289|Q9SFH8 (T6C23.7)HYPOTHETICAL 88.6 KDA PROTEIN.[Arab... 33
4.1
tr|AC010924|Q9S9M6 (T24D18.19)T24D18.19 PROTEIN.[Arabidopsis th... 33
5.4
tr|AC010924|Q9S9M4 (T24D18.21)T24D18.21 PROTEIN.[Arabidopsis th... 32
7.1
tr|AF020904|O16859 (MAFP3E)AGGREGATION FACTOR PROTEIN 3, FORM E... 32
9.3
tr|AB037819|Q9P2E9 (KIAA1398)KIAA1398 PROTEIN (FRAGMENT).[Homo ... 32
9.3
tr|AE004191|Q9KSZ6 (VC1110)SERYL-TRNA SYNTHETASE.[Vibrio cholerae] 32
9.3
tn|AL132765|CAC10588 (RRBP1)BA462D18.3.2 (ribosome binding prot... 32
9.3
>sp|P53044|UFD1_YEAST (UFD1..)UBIQUITIN FUSION DEGRADATION PROTEIN 1
(UB FUSION PROTEIN 1) (POLYMERASE-INTERACTING PROTEIN
3).[Saccharomyces cerevisiae]
Length = 361
Score = 655 bits
(1672), Expect = 0.0
Identities = 329/347 (94%), Positives = 329/347
(94%)
Query: 15
VNMPQTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTAN 74
VNMPQTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTAN
Sbjct: 15
VNMPQTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTAN 74
Query:
75 ETGRVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDF 134
ETGRVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDF
Sbjct: 75
ETGRVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDF 134
Query:
135 LDISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVT 194
LDISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVT
Sbjct: 135
LDISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVT 194
Query:
195 DFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNK 254
DFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNK
Sbjct: 195
DFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNK 254
Query:
255 LSKFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEES 314
LSKFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEES
Sbjct: 255
LSKFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEES 314
Query:
315 AAGSKSSEQNFQGQGISLRKSNXXXXXXXXXXXXXXXXXXPEVIEID 361
AAGSKSSEQNFQGQGISLRKSN PEVIEID
Sbjct: 315
AAGSKSSEQNFQGQGISLRKSNKRKTKSDHDSSKSKAPKSPEVIEID 361
>tr|AJ005825|O42915 (UFD1..)UBIQUITIN FUSION DEGRADATION PROTEIN 1
(UB FUSION PROTEIN 1).[Schizosaccharomyces pombe]
Length = 342
Score = 264 bits (667), Expect = 1e-69
Identities = 145/320 (45%),
Positives = 197/320 (61%), Gaps = 24/320 (7%)
Query: 16
NMPQTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANE 75
N+ Q F+ +RCYP+AM+ R + N+GGK+ LPPSAL KLS LN+ YPMLF
Sbjct: 27
NVNQRFDTRYRCYPVAMIPGEERPN-VNYGGKVILPPSALEKLSRLNVSYPMLFDFENEA 85
Query:
76 TGRVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFL 135
+ THGGVLEFIAEEGRVYLP WMM TL ++PG L+++ +TD+ G +VKL+PQSV+FL
Sbjct: 86
AEKKTHGGVLEFIAEEGRVYLPYWMMTTLSLEPGDLVRVINTDIAQGSYVKLQPQSVNFL 145
Query:
136 DISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTD 195
DI+D +AVLEN LRNFSTLT D+ EI YN + ++IK+++V+P+ S + V+ETDLV D
Sbjct: 146
DITDHRAVLENALRNFSTLTKSDIFEILYNDQVYQIKVIDVQPDDSRHVVSVVETDLVVD 205
Query:
196 FAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKL 255
F PP+GY E + Q +++N G QG+M T+I Y + + L
Sbjct: 206
FDPPIGYEE-------SLQKNKQQNIAGV----------QGTMVTKIGYDELVRQGDSNL 248
Query:
256 SKFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESA 315
K G G ++GK PK ++ D + PA L LP G FFG+P P +E+S
Sbjct: 249
MK--GTGTKLNGKEVAEVPKINLLD--VEKQECPAPLILPLGTYFFGYPYKAPSIEEDSK 304
Query:
316 AGSKSSEQNFQGQGISLRKS 335
+ F+G G SLR S
Sbjct: 305
KDPNLFK--FEGAGTSLRAS 322
>tn|AF234601|AAG27535 UFD1.[Rattus norvegicus]
Length = 307
Score = 204 bits (514), Expect = 1e-51
Identities = 128/320 (40%),
Positives = 179/320 (55%), Gaps = 42/320 (13%)
Query: 21
FEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVT 80
F +RC+ ++M+ + D GGKI +PPSAL +LS LNI YPMLFKLT + R+T
Sbjct: 19
FSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMT 78
Query:
81 HGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDP 140
H GVLEF+A+EG YLP WMM+ L ++ G L+Q+ S ++ + + K +PQS DFLDI++P
Sbjct: 79
HCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNP 138
Query:
141 KAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPV 200
KAVLEN LRNF+ LT DVI I+YN K ++++++E KP+ K++ +IE D+ DF P+
Sbjct: 139
KAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPD---KAVSIIECDMNVDFDAPL 195
Query:
201 GYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVG 260
GY EP+ + Q +E S+ D++G A F G
Sbjct: 196
GYKEPE----RPVQHEE-------------------SIEGEADHSGYAGEV--GFRAFSG 230
Query:
261 QGQNISGKAPKAEPK-QDIK--DMKITFDGEPAKLDLPEGQLFF---GFPMVLPKEDEES 314
G + GK EP IK D+K KL G++ F PMV K+ EE
Sbjct: 231
SGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKL----GKITFIRNSRPMV--KKVEED 284
Query:
315 AAGSKSSEQNFQGQGISLRK 334
AG + F G+G SLRK
Sbjct: 285
EAGGRFVA--FSGEGQSLRK 302
>sp_vs|Q92890-01|Q92890 SHORT ISOFORM OF Q92890[Homo sapiens]
Length = 307
Score = 203 bits (512), Expect = 2e-51
Identities =
122/320 (38%), Positives = 178/320 (55%), Gaps = 42/320 (13%)
Query: 21
FEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVT 80
F +RC+ ++M+ + D GGKI +PPSAL +LS LNI YPMLFKLT + R+T
Sbjct: 19
FSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMT 78
Query:
81 HGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDP 140
H GVLEF+A+EG YLP WMM+ L ++ G L+Q+ S ++ + + K +PQS DFLDI++P
Sbjct: 79
HCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNP 138
Query:
141 KAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPV 200
KAVLEN LRNF+ LT DVI I+YN K ++++++E KP+ K++ +IE D+ DF P+
Sbjct: 139
KAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPD---KAVSIIECDMNVDFDAPL 195
Query:
201 GYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVG 260
GY EP+ +Q + ++++ G+ D++G A F G
Sbjct: 196
GYKEPE------RQVQHEESTEGEA-----------------DHSGYAGEL--GFRAFSG 230
Query:
261 QGQNISGKAPKAEPKQ------DIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEES 314
G + GK EP DIK ++ + K+ F L K+ EE
Sbjct: 231
SGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKI------TFIRNSRPLVKKVEED 284
Query:
315 AAGSKSSEQNFQGQGISLRK 334
AG + F G+G SLRK
Sbjct: 285
EAGGRFVA--FSGEGQSLRK 302
>sp|P70362|UFD1_MOUSE (UFD1L)UBIQUITIN FUSION DEGRADATION PROTEIN 1
HOMOLOG (UB FUSION PROTEIN 1).[Mus musculus]
Length = 307
Score = 201 bits (506), Expect = 9e-51
Identities = 122/320 (38%),
Positives = 174/320 (54%), Gaps = 42/320 (13%)
Query: 21
FEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVT 80
F +RC+ ++M+ + D GGKI +PPSAL +LS LNI YPMLFKLT + R+T
Sbjct: 19
FSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMT 78
Query:
81 HGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDP 140
H GVLEF+A+EG YLP WMM+ L ++ G L+Q+ S ++ + + K +PQS DFLDI++P
Sbjct: 79
HCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNP 138
Query:
141 KAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPV 200
KAVLEN LRNF+ +T DVI I+YN K ++++++E KP+ K++ +IE D+ DF P+
Sbjct: 139
KAVLENALRNFACMTTGDVIAINYNEKIYELRVMETKPD---KAVSIIECDMNVDFDAPL 195
Query:
201 GYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVG 260
GY EP + Q +E S+ D++G A F G
Sbjct: 196
GYKEPK----RPVQHEE-------------------SIEGEADHSGYAGEV--GFRAFSG 230
Query:
261 QGQNISGKAPKAEPKQ------DIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEES 314
G + GK EP DIK ++ + K+ F L K+ EE
Sbjct: 231
SGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKI------TFIRNSRPLVKKVEED 284
Query:
315 AAGSKSSEQNFQGQGISLRK 334
AG + F G+G SLRK
Sbjct: 285
EAGGRFVA--FSGEGQSLRK 302
>tn|AF233524|AAG25922 Ubiquitin fusion degradation 1-like protein
(Fragment).[Xenopus laevis]
Length = 305
Score = 200 bits
(504), Expect = 2e-50
Identities = 116/286 (40%), Positives = 160/286
(55%), Gaps = 18/286 (6%)
Query: 21
FEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVT 80
F +RCY ++M+ + D GGKI +PPSAL +LS LNI YPMLFKLT + R+T
Sbjct: 19
FSTQYRCYSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMT 78
Query:
81 HGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDP 140
H GVLEF+A+EG YLP WMM+ L ++ LLQ+ S ++ + + K +PQS DFLDI++P
Sbjct: 79
HCGVLEFVADEGVCYLPHWMMQNLLLEEEGLLQVESVNLQVATYSKFQPQSPDFLDITNP 138
Query:
141 KAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPV 200
KAVLEN LRNF+ LT DVI I+YN K ++++++E KP+ K++ +IE D+ DF P+
Sbjct: 139
KAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPD---KAVSIIECDMNVDFDAPL 195
Query:
201 GYVEPDYKALKAQQ------------DKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIA 248
GY EPD + +Q D + G G LD G G + + GI
Sbjct: 196
GYKEPDRHTQQEEQTEIEPDHSEYAADLGFRAFSGSGNRLDGKKKGIGPSPSPLKPEGIR 255
Query:
249 NSSRNKLSKFVGQGQNISGKAP--KAEPKQDIKDMKITFDGEPAKL 292
N
K +G I P K + D + I F GE L
Sbjct: 256
RGIPNYDFK-IGTITFIRNSRPQTKKVEEDDTANQFIAFSGEGQSL 300
>sp|Q9VTF9|UFD1_DROME (CG6233)UBIQUITIN FUSION DEGRADATION PROTEIN 1
HOMOLOG (UB FUSION PROTEIN 1).[Drosophila melanogaster]
Length =
316
Score = 196 bits (492), Expect = 4e-49
Identities = 120/324
(37%), Positives = 176/324 (54%), Gaps = 31/324 (9%)
Query: 19
QTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGR 78
+ F ++C+ ++M+ R D GGKI +PPSAL L+ LN+ YPMLFKLT + R
Sbjct: 15
RNFHANYKCFSVSMLPGNERTD-VEKGGKIIMPPSALDTLTRLNVEYPMLFKLTNVKKSR 73
Query:
79 VTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDIS 138
+H GVLEF+A+EG+ YLP WMME L + G +L I S +P+ F K +P S DFLDI+
Sbjct: 74
SSHAGVLEFVADEGKCYLPHWMMENLLLGEGDILNIESVSLPVATFSKFQPHSTDFLDIT 133
Query:
139 DPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAP 198
+PKAVLEN LRNF+ LT DVI I YN K +++ +LE KP ++ +IE D+ +F
Sbjct: 134
NPKAVLENALRNFACLTRGDVIAIKYNKKVYELCVLETKP---GNAVSIIECDMNVEFEA 190
Query:
199 PVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMST-RIDYAGIANSSRNK-LS 256
PVGY +D + + G GQ QG+ T + AG N+ + +
Sbjct: 191
PVGY-----------KDHSETQASGSGQ--------QGAAGTVGGEIAGATNAILEEVVE 231
Query:
257 KFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFG---FPMVLPKEDEE 313
F G G + GK K + K+ G P D G + F P+ ++++
Sbjct: 232
TFKGSGVRLDGKKKKESQLETPVVKKVLARGVP-DYDFQFGLIRFDRNIRPISDRSQEDD 290
Query:
314 SAAGS--KSSEQNFQGQGISLRKS 335
+ AG+ S ++F G G S++K+
Sbjct: 291 AVAGNADASDAESFHGTGFSMKKT 314
>tr|AC006841|Q9SJV0 (AT2G21270)PUTATIVE UBIQUITIN FUSION-DEGRADATION
PROTEIN.[Arabidopsis thaliana]
Length = 320
Score = 192
bits (484), Expect = 3e-48
Identities = 124/310 (40%), Positives =
173/310 (55%), Gaps = 36/310 (11%)
Query: 20
TFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRV 79
TFE+ +RCYP + ++ K G KI +PPSAL +L+ L+I YPMLF+L RV
Sbjct: 13
TFEQSYRCYPASFID----KPQLESGDKIIMPPSALDRLASLHIDYPMLFELRNAGIERV 68
Query:
80 THGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISD 139
TH GVLEFIAEEG +Y+P WMM+ L +Q G ++++ + +P G +VKL+P + DFLDIS+
Sbjct: 69
THCGVLEFIAEEGMIYMPYWMMQNLLLQEGDIVRVRNVTLPKGTYVKLQPHTTDFLDISN 128
Query:
140 PKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPP 199
PKA+LE LRN+S LT D I + YN K + I I+E KP + +I +IETD DFAPP
Sbjct: 129
PKAILETALRNYSCLTSGDSIMVPYNNKKYFIDIVETKP---ANAISIIETDCEVDFAPP 185
Query:
200 VGYVEPD-----------YKALKAQQDKEKK-NSF-GKGQVLD---------PSVLGQGS 237
+ Y EP+ KA + + E K N F G G+ LD P+ +G
Sbjct: 186
LDYKEPERPTAPSAAKGPAKAEEVVDEPEPKFNPFTGSGRRLDGRPLAYEPAPASSSKGK 245
Query:
238 MSTRIDYAGIANSSRNKLSKFVGQGQNISG----KAPK-AEPKQDIKDMKITFDGEPAKL 292
+ G ++ + + QG+ + G +APK A PK + K T E K
Sbjct: 246
QPVVANGNGQSSVASSSEKATRAQGKLVFGANGNRAPKEAAPK--VGAAKETKKEEQEKK 303
Query:
293 DLPEGQLFFG 302
D P+ Q F G
Sbjct: 304 DEPKFQAFSG 313
>tr|AL035679|Q9SVK0 (F19H22.30..)PUTATIVE UBIQUITIN-DEPENDENT
PROTEOLYTIC PROTEIN.[Arabidopsis thaliana]
Length = 315
Score = 190 bits (478), Expect = 2e-47
Identities = 104/256 (40%),
Positives = 145/256 (56%), Gaps = 30/256 (11%)
Query: 20
TFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRV 79
TFE+ +RCYP + ++ K G KI +PPSAL +L+ L I YPMLF+L T
Sbjct: 12
TFEQTYRCYPSSFID----KPQIESGDKIIMPPSALDRLASLQIDYPMLFELRNASTDSF 67
Query:
80 THGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISD 139
+H GVLEFIAEEG +Y+P WMM+ L +Q G ++++ + +P G +VKL+P + DFLDI++
Sbjct: 68
SHCGVLEFIAEEGVIYIPYWMMQNLLLQEGDMVRVRNVTLPKGTYVKLQPHTTDFLDIAN 127
Query:
140 PKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPP 199
PKA+LE LRN+S LTV D I + YN K + I I+E KP S I +IETD DFAPP
Sbjct: 128
PKAILETALRNYSCLTVGDSIMVPYNNKKYFIDIVEAKP---SNGISIIETDCEVDFAPP 184
Query:
200 VGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFV 259
+ Y EP+ A E K + +D A + K + F
Sbjct: 185
LDYKEPERPVAPAPAKGEAK-------------------AKEVDVA----EAEPKFNPFT 221
Query:
260 GQGQNISGKAPKAEPK 275
G G+ + G+ EP+
Sbjct: 222
GSGRRLDGRPLSYEPQ 237
>sp|Q92890|UFD1_HUMAN (UFD1L)UBIQUITIN FUSION DEGRADATION PROTEIN 1
HOMOLOG (UB FUSION PROTEIN 1).[Homo sapiens]
Length = 343
Score = 185 bits (466), Expect = 4e-46
Identities = 124/356 (34%),
Positives = 179/356 (49%), Gaps = 78/356 (21%)
Query: 21
FEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVT 80
F +RC+ ++M+ + D GGKI +PPSAL +LS LNI YPMLFKLT + R+T
Sbjct: 19
FSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMT 78
Query:
81 HGGVLEFIAEEGRVYLPQWMM-------------ETLGIQ-------------------- 107
H GVLEF+A+EG YLP WMM ET+ +Q
Sbjct: 79
HCGVLEFVADEGICYLPHWMMQNLLLEEDGLVQLETVNLQVATYSKSKFCYLPHWMMQNL 138
Query:
108 ---PGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVIEISY 164
G L+Q+ S ++ + + K +PQS DFLDI++PKAVLEN LRNF+ LT DVI I+Y
Sbjct: 139
LLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINY 198
Query:
165 NGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGK 224
N K ++++++E KP+ K++ +IE D+ DF P+GY EP+ +Q + ++++ G+
Sbjct: 199
NEKIYELRVMETKPD---KAVSIIECDMNVDFDAPLGYKEPE------RQVQHEESTEGE 249
Query:
225 GQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKAPKAEPKQ------DI 278
D++G A F G G + GK EP DI
Sbjct: 250
A-----------------DHSGYAGEL--GFRAFSGSGNRLDGKKKGVEPSPSPIKPGDI 290
Query:
279 KDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSEQNFQGQGISLRK 334
K ++ + K+ F L K+ EE AG + F G+G SLRK
Sbjct: 291
KRGIPNYEFKLGKI------TFIRNSRPLVKKVEEDEAGGRFVA--FSGEGQSLRK 338
>sp|Q19584|UFD1_CAEEL (F19B6.2)UBIQUITIN FUSION DEGRADATION PROTEIN
1 HOMOLOG (UB FUSION PROTEIN 1).[Caenorhabditis elegans]
Length
= 342
Score = 170 bits (427), Expect = 2e-41
Identities = 123/327
(37%), Positives = 174/327 (52%), Gaps = 22/327 (6%)
Query: 21
FEEFFRCY-PIAMMNDRIRK-DDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGR 78
+++ F Y P+ + N K + N+GGKI LP SAL+ L NI PMLFKLT R
Sbjct: 21
YDQTFVVYGPVFLPNATQSKISEINYGGKILLPSSALNLLMQYNIPMPMLFKLTNMAVQR 80
Query:
79 VTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDIS 138
VTH GVLEF A EG+ LP WMM+ LG+ G ++I S +P F KL+P S++FL+I+
Sbjct: 81
VTHCGVLEFSAPEGQAILPLWMMQQLGLDDGDTIRIESATLPKATFAKLKPMSLEFLNIT 140
Query:
139 DPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAP 198
+PKAVLE LR ++ LT +D I SY G+T + ++++KP + S+C+IE D+ DF P
Sbjct: 141
NPKAVLEVELRKYACLTKNDRIPTSYAGQTLEFLVVDLKP---ANSVCIIECDVNLDFDP 197
Query:
199 PVGYVEPDYK---ALKAQQDKEKKNSF-GKGQVLDPSVLGQGSMSTRI-DYAGIANSSRN 253
P GYVE + A+ A+ ++F G GQ S G G +T + AG +
Sbjct: 198
PEGYVEQPRQVTPAVTAKPPAPDASAFIGAGQKAGGSG-GTGQNATSVFGGAGRRLDGKK 256
Query:
254 KLSKFV----GQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPM--VL 307
K S V G G + S AP A I + + D +P ++ L + + VL
Sbjct: 257
KPSSSVSLSDGTGVSTSNAAPVANDLPAIPPVVVNEDYKPGRVSF----LRYDYKRVDVL 312
Query:
308 PKEDEESAAGSKSSEQN-FQGQGISLR 333
KE E A N F+G
+LR
Sbjct: 313 EKELREKEASKAGQPSNVFRGGNRTLR 339
>tr|AC005315|O81075 (T9I4.15)PUTATIVE UBIQUITIN.[Arabidopsis
thaliana]
Length = 292
Score = 169 bits (425), Expect =
3e-41
Identities = 99/263 (37%), Positives = 144/263 (54%), Gaps = 31/263
(11%)
Query: 47
KIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVLEFIAEEGRVYLPQWMMETLGI 106
+ +PPSAL +L+ L+I YPMLF+L+ + +H GVLEF A+EG VYLP WMM+ + +
Sbjct: 10
QFIMPPSALDRLASLHIEYPMLFQLSNVSVEKTSHCGVLEFTADEGLVYLPYWMMQNMSL 69
Query:
107 QPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVIEISYNG 166
+ G ++Q+ + + G ++KL+P + DFLDIS+PKA+LE LR++S LT D I + YN
Sbjct: 70
EEGDVMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILETTLRSYSCLTTGDTIMVPYNN 129
Query:
167 KTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQ 226
K + I ++E KP S ++ +IETD DFAPP+ Y EP+ K Q
Sbjct: 130
KQYYINVVEAKP---SSAVSIIETDCEVDFAPPLDYKEPE-----------------KPQ 169
Query:
227 VLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKA-PKAEP---KQDIKDMK 282
L PS ++ S K + F G G+ + GKA + EP KQ K +
Sbjct: 170
KLTPS----NKRPLQVKEEEEPASKVPKFTPFTGSGKRLDGKAQTQTEPEDTKQQEKPTE 225
Query:
283 ITFDGEPAKLDLP---EGQLFFG 302
D E + P G+L FG
Sbjct:
226 NGKDDEKLSVTTPRQISGKLVFG 248
>tr|Z69635|Q9U3I6 (F19B6.2B)F19B6.2B PROTEIN.[Caenorhabditis
elegans]
Length = 336
Score = 169 bits (425), Expect =
3e-41
Identities = 118/305 (38%), Positives = 164/305 (53%), Gaps =
20/305 (6%)
Query: 41
DANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVLEFIAEEGRVYLPQWM 100
+ N+GGKI LP SAL+ L NI PMLFKLT RVTH GVLEF A EG+ LP WM
Sbjct: 37
EINYGGKILLPSSALNLLMQYNIPMPMLFKLTNMAVQRVTHCGVLEFSAPEGQAILPLWM 96
Query:
101 METLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVI 160
M+ LG+ G ++I S +P F KL+P S++FL+I++PKAVLE LR ++ LT +D I
Sbjct: 97
MQQLGLDDGDTIRIESATLPKATFAKLKPMSLEFLNITNPKAVLEVELRKYACLTKNDRI 156
Query:
161 EISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYK---ALKAQQDKE 217
SY G+T + ++++KP + S+C+IE D+ DF PP GYVE + A+ A+
Sbjct: 157
PTSYAGQTLEFLVVDLKP---ANSVCIIECDVNLDFDPPEGYVEQPRQVTPAVTAKPPAP 213
Query:
218 KKNSF-GKGQVLDPSVLGQGSMSTRI-DYAGIANSSRNKLSKFV----GQGQNISGKAPK 271
++F G GQ S G G +T + AG + K S V G G + S AP
Sbjct: 214
DASAFIGAGQKAGGSG-GTGQNATSVFGGAGRRLDGKKKPSSSVSLSDGTGVSTSNAAPV 272
Query:
272 AEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPM--VLPKEDEESAAGSKSSEQN-FQGQ 328
A I + + D +P ++ L + + VL KE E A N F+G
Sbjct: 273
ANDLPAIPPVVVNEDYKPGRVSF----LRYDYKRVDVLEKELREKEASKAGQPSNVFRGG 328
Query:
329 GISLR 333
+LR
Sbjct: 329 NRTLR 333
>tr|AF083031|Q9SEV9 (UFD1)UBIQUITIN FUSION DEGRADATION
PROTEIN.[Guillardia theta]
Length = 175
Score = 106 bits
(262), Expect = 4e-22
Identities = 60/167 (35%), Positives = 94/167
(55%), Gaps = 7/167 (4%)
Query: 26
RCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVL 85
+ YP++ I K G KI LP S L+ L+ + P++F++ + + H GV
Sbjct: 9
KTYPLSF----IGKSFLENGDKIVLPQSILNYLNQNDDLNPIIFEILNLDNNKKCHCGVY 64
Query:
86 EFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLE 145
EF +++G Y+P WM + L I GS L + G F+K++PQ +F IS+PKA+LE
Sbjct: 65
EFTSDDGCAYIPYWMFKNLEINEGSPLCFIQKCLEKGYFLKIQPQQKEFFQISNPKAILE 124
Query:
146 NVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDL 192
LR
+++LT + I I YN + + I+EVKP +I +I+TDL
Sbjct: 125
LNLRKYTSLTKKNTISIEYNNNIYWLNIVEVKP---GNAINIIDTDL 168
>tr|Z97338|O23395 (AT4G15420)SIMILAR TO UFD1 PROTEIN (UFD1 LIKE
PROTEIN).[Arabidopsis thaliana]
Length = 778
Score = 102
bits (252), Expect = 6e-21
Identities = 65/191 (34%), Positives = 107/191
(55%), Gaps = 18/191 (9%)
Query: 45
GGKIFLPPSALSKLSMLNI--RYPMLFKLTA---NETGRVTHGGVLEFIAEEGRVYLPQ- 98
G KI LPPS ++LS + P+ F+L+ + + TH GVLEF AE+G + LP
Sbjct: 308
GDKIKLPPSCFTELSDQGAFDKGPLYFELSVVDHADNKKTTHSGVLEFTAEDGTIGLPPH 367
Query:
99 -W--MMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLT 155
W + T L++I +P G + KL+P ++ F D+ + KA+LE +LR +TL+
Sbjct: 368
VWSNLFSTHDPMDVPLVEIRYIRLPKGSYAKLQPDNLGFSDLPNHKAILETILRQHATLS 427
Query:
156 VDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQD 215
+DDV+ ++Y ++K+++LE++P + SI V+ETD+ D V PD + + Q
Sbjct: 428
LDDVLLVNYGQVSYKLQVLELRP---ATSISVLETDIEVDI------VSPDIVSDQPNQH 478
Query:
216 KEKKNSFGKGQ 226
K +GK +
Sbjct: 479 VLKPLQYGKSE 489
>tn|AF315735|AAG31651 PRLI-interacting factor K
(Fragment).[Arabidopsis thaliana]
Length = 574
Score = 102
bits (252), Expect = 6e-21
Identities = 65/191 (34%), Positives = 107/191
(55%), Gaps = 18/191 (9%)
Query: 45
GGKIFLPPSALSKLSMLNI--RYPMLFKLTA---NETGRVTHGGVLEFIAEEGRVYLPQ- 98
G KI LPPS ++LS + P+ F+L+ + + TH GVLEF AE+G + LP
Sbjct: 104
GDKIKLPPSCFTELSDQGAFDKGPLYFELSVVDHADNKKTTHSGVLEFTAEDGTIGLPPH 163
Query:
99 -W--MMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLT 155
W + T L++I +P G + KL+P ++ F D+ + KA+LE +LR +TL+
Sbjct: 164
VWSNLFSTHDPMDVPLVEIRYIRLPKGSYAKLQPDNLGFSDLPNHKAILETILRQHATLS 223
Query:
156 VDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQD 215
+DDV+ ++Y ++K+++LE++P + SI V+ETD+ D V PD + + Q
Sbjct: 224
LDDVLLVNYGQVSYKLQVLELRP---ATSISVLETDIEVDI------VSPDIVSDQPNQH 274
Query:
216 KEKKNSFGKGQ 226
K +GK +
Sbjct: 275 VLKPLQYGKSE 285
>tr|AE001179|O51745 (BB0805)POLYRIBONUCLEOTIDE
NUCLEOTIDYLTRANSFERASE (PNPA).[Borrelia burgdorferi]
Length =
722
Score = 34.8 bits (78), Expect = 1.4
Identities = 32/88 (36%),
Positives = 44/88 (49%), Gaps = 13/88 (14%)
Query: 217
EKKNSFGKGQVLDPSVLGQGSMSTRID-----YAGIANSSRNKLSKFVGQGQNISGKA-- 269
+K NSFG L P+ +G +STR+ Y + N+ S+F G G+NI G A
Sbjct: 630
KKINSFGAFIELTPAK--EGFLSTRLKPRDSKYGSGRFGNSNRYSRFGGGGENIRGNAGL 687
Query:
270 ---PKAEPKQDIKDMKITFDGEPAKLDL 294
PK E Q IK +KI +
K+DL
Sbjct: 688 VRPPKLEEGQRIK-VKIIDIDKFGKIDL 714
>tr|AC010793|Q9MA08 F20B17.10.[Arabidopsis thaliana]
Length =
1487
Score = 33.6 bits (75), Expect = 3.2
Identities = 19/65 (29%),
Positives = 36/65 (55%), Gaps = 2/65 (3%)
Query: 175
EVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLG 234
+V +S+++ V T L T + VGY++P+Y ++ Q +K + + G VL + G
Sbjct: 574
KVSDFGTSRTVTVDHTHLTTVVSGTVGYMDPEY--FQSSQFTDKSDVYSFGVVLAELITG 631
Query:
235 QGSMS 239
+ S+S
Sbjct: 632 EKSVS 636
>tr|AC010924|Q9S9M2 (T24D18.23)T24D18.23 PROTEIN.[Arabidopsis
thaliana]
Length = 700
Score = 33.6 bits (75), Expect = 3.2
Identities = 17/50 (34%), Positives = 26/50 (52%)
Query: 174
LEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFG 223
++V
+S+S+ + +T L T A GYV+P+Y DK SFG
Sbjct: 502
VKVSDFGTSRSVTIDQTHLTTQVAGTFGYVDPEYFQSSKFTDKSDVYSFG 551
>tr|Z97182|O07779 (RV0599C..)HYPOTHETICAL 8.2 KDA
PROTEIN.[Mycobacterium tuberculosis]
Length = 78
Score =
33.6 bits (75), Expect = 3.2
Identities = 16/45 (35%), Positives = 28/45
(61%), Gaps = 5/45 (11%)
Query: 92
GRVYLPQWMMETLGIQPGSLLQISSTD-----VPLGQFVKLEPQS 131
GR+ +P+ + E
LG+QPGS ++IS +P G+ +LE ++
Sbjct: 9
GRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEEN 53
>tr|AC013289|Q9SFH8 (T6C23.7)HYPOTHETICAL 88.6 KDA
PROTEIN.[Arabidopsis thaliana]
Length = 792
Score = 33.2
bits (74), Expect = 4.1
Identities = 19/65 (29%), Positives = 36/65
(55%), Gaps = 2/65 (3%)
Query: 175
EVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLG 234
+V +S+++ V T L T + VGY++P+Y ++ Q +K + + G VL + G
Sbjct: 588
KVSDFGTSRTVTVDHTHLTTVVSGTVGYMDPEY--FQSSQFTDKSDVYSFGVVLVELITG 645
Query:
235 QGSMS 239
+ S+S
Sbjct: 646 EKSIS 650
>tr|AC010924|Q9S9M6 (T24D18.19)T24D18.19 PROTEIN.[Arabidopsis
thaliana]
Length = 1016
Score = 32.8 bits (73), Expect =
5.4
Identities = 21/67 (31%), Positives = 33/67 (48%), Gaps = 2/67 (2%)
Query: 175 EVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLG
234
+V +S+SI + +T L T A GY++P+Y DK SF G VL +
G
Sbjct: 948 KVSDFGTSRSITIAQTHLTTLVAGTFGYMDPEYFLSSQYTDKSDVYSF--GVVLVELITG
1005
Query: 235 QGSMSTR 241
+ +S +
Sbjct: 1006 EKPLSRK
1012
>tr|AC010924|Q9S9M4 (T24D18.21)T24D18.21 PROTEIN.[Arabidopsis
thaliana]
Length = 664
Score = 32.5 bits (72), Expect = 7.1
Identities = 18/61 (29%), Positives = 33/61 (53%), Gaps = 2/61 (3%)
Query: 175 EVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLG
234
+V +S+S+ + +T L T A GYV+P+Y ++ + EK + + G VL +
G
Sbjct: 472 KVSDFGTSRSVTIDQTHLTTQVAGTFGYVDPEY--FQSSKFTEKSDVYSFGVVLVELLTG
529
Query: 235 Q 235
+
Sbjct: 530 E 530
>tr|AF020904|O16859 (MAFP3E)AGGREGATION F
ACTOR PROTEIN 3, FORM E
(FRAGMENT).[Microciona prolifera]
Length = 825
Score = 32.1 bits (71), Expect = 9.3
Identities = 24/91
(26%), Positives = 44/91 (47%), Gaps = 11/91 (12%)
Query: 88
IAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDIS-----DPKA 142
+AEEG Y P + T+ I P S ++ ++++ L LE + L I+ D +
Sbjct: 329
MAEEGEDYDPANVPSTVTIAPNSSMECFTSNLVLDDGESLEEDEIVVLSIAAVDPDDSRI 388
Query:
143 VLENVLRNFSTLTVDD----VIEISYNGKTF 169
++ NV N + + +DD VI
+ + T+
Sbjct: 389 IVSNV--NVTVVIIDDDELQVITVQFEQSTY 417
>tr|AB037819|Q9P2E9 (KIAA1398)KIAA1398 PROTEIN (FRAGMENT).[Homo
sapiens]
Length = 1456
Score = 32.1 bits (71), Expect = 9.3
Identities = 22/76 (28%), Positives = 33/76 (42%)
Query: 260
GQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSK 319
G+G I GK + Q K IT G+ A+ EG+ G P K D + G K
Sbjct: 785
GEGAPIQGKKADSVANQGTKVEGITNQGKKAEGSPSEGKKAEGSPNQGKKADAAANQGKK 844
Query:
320 SSEQNFQGQGISLRKS 335
+ + QG+ + +S
Sbjct: 845
TESASVQGRNTDVAQS 860
>tr|AE004191|Q9KSZ6 (VC1110)SERYL-TRNA SYNTHETASE.[Vibrio cholerae]
Length = 435
Score = 32.1 bits (71), Expect = 9.3
Identities = 29/124
(23%), Positives = 50/124 (39%), Gaps = 5/124 (4%)
Query: 206
DYKALKAQQDKEKKNSFGKGQVLDPSV---LGQGSMSTRIDYAGIANSSRNKLSKFVGQG 262
D K L+ + D+ +G LD L + S +++ + S+RN +SK +GQ
Sbjct: 3
DSKLLRTELDETAAKLARRGFKLDVETIRTLEEQRKSIQVEVENL-QSTRNSISKQIGQL 61
Query:
263 QNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSE 322
KA KQ I + D + +LD QL +P +++ K
Sbjct: 62
MASGDKAGAEAVKQQIGTLGDDLDAKKVELDAVMAQL-DAITQTVPNIPDDAVPNGKDDS 120
Query:
323 QNFQ 326
+N +
Sbjct: 121 ENVE 124
>tn|AL132765|CAC10588 (RRBP1)BA462D18.3.2 (ribosome binding protein
1 (dog 180 kDa homolog)) (Fragment).[Homo sapiens]
Length =
1208
Score = 32.1 bits (71), Expect = 9.3
Identities = 22/76 (28%),
Positives = 33/76 (42%)
Query: 260
GQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSK 319
G+G I GK + Q K IT G+ A+ EG+ G P K D + G K
Sbjct: 536
GEGAPIQGKKADSVANQGTKVEGITNQGKKAEGSPSEGKKAEGSPNQGKKADAAANQGKK 595
Query:
320 SSEQNFQGQGISLRKS 335
+ + QG+ + +S
Sbjct: 596
TESASVQGRNTDVAQS 611
Database: swiss_nr
Posted date: Jan 26,
2001 6:11 AM
Number of letters in database: 33,669,062
Number of
sequences in database: 92,100
Database: swiss_varsplic_nr
Posted date: Jan 26, 2001 6:11 AM
Number of letters in database:
1,587,947
Number of sequences in database: 2435
Database:
trembl_new_nr
Posted date: Jan 26, 2001 6:12 AM
Number of letters
in database: 15,364,861
Number of sequences in database: 46,874
Database: trembl_nr
Posted date: Jan 26, 2001 6:16 AM
Number of
letters in database: 115,571,020
Number of sequences in database:
358,354
Database: trembl_varsplic_nr
Posted date: Jan 26, 2001
6:17 AM
Number of letters in database: 391,702
Number of sequences in
database: 522
Lambda K H
0.315 0.136 0.382
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix:
BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB:
107471254
Number of Sequences: 500285
Number of extensions: 4473755
Number of successful extensions: 7695
Number of sequences better than 10.0: 27
N
umber of HSP's better than 10.0 without gapping: 20
Number of HSP's
successfully gapped in prelim test: 7
Number of HSP's that attempted
gapping in prelim test: 7650
Number of HSP's gapped (non-prelim): 32
length of query: 361
length of database: 166,584,592
effective HSP
length: 57
effective length of query: 304
effective length of database:
138,068,347
effective search space: 41972777488
effective search space
used: 41972777488
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (22.0 bits)
S2: 71 (32.1 bits)
Thank
you for using BLAST2.0